Recombinant Human CA199 (FUT3) Protein (His Tag)

Product Specifications

Background

May catalyze alpha-1,3 and alpha-1,4 glycosidic linkages involved in the expression of Vim-2, Lewis A, Lewis B, sialyl Lewis X and Lewis X/SSEA-1 antigens. May be involved in blood group Lewis determination, Lewis-positive (Le+) individuals have an active enzyme while Lewis-negative (Le-) individuals have an inactive enzyme. Also acts on the corresponding 1,4-galactosyl derivative, forming 1,3-L-fucosyl links.

Abbreviation

CA199

Synonyms

Lewis FT; Blood group Lewis alpha-4-fucosyltransferase; FT3B; FUT3; FucT-III; Fucosyltransferase 3; Fucosyltransferase III; Galactoside 3 (4) -L-fucosyltransferase; LE

UniProt

P21217

Accession Number

P21217-1

Expression System

E.coli

Tag

N-His

Sequence

Val35-Thr361

Field of Research

Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 80% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.