Recombinant Human BRCA2 Protein (His Tag)
Product Specifications
Background
Inherited mutations in BRCA1 and this gene, BRCA2, confer increased lifetime risk of developing breast or ovarian cancer. Both BRCA1 and BRCA2 are involved in maintenance of genome stability, specifically the homologous recombination pathway for double-strand DNA repair. The BRCA2 protein contains several copies of a 70 aa motif called the BRC motif, and these motifs mediate binding to the RAD51 recombinase which functions in DNA repair. BRCA2 is considered a tumor suppressor gene, as tumors with BRCA2 mutations generally exhibit loss of heterozygosity (LOH) of the wild-type allele.
Abbreviation
BRCA2
Synonyms
BRCA 2; BRCA1/BRCA2 containing complex subunit 2; BRCA2; BRCC 2; BRCC2; Brca2; Breast Cancer 2 t; Breast and ovarian cancer susceptibility gene early onset; Breast cancer 2 early onset; DNA repair associated; breast and ovarian cancer susceptibility protein 2
UniProt
P51587
Accession Number
P51587-1
Expression System
E.coli
Tag
N-His
Sequence
Thr2350-Val2545
Applications
Immunogen
Field of Research
Epigenetics and Nuclear Signaling, Cancer
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 85% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/hzey9c
Product MSDS
https://789.bio/eb/C8CxLC
Calculated Molecular Weight
22.1 kDa
Observed Molecular Weight
25.4 kDa
Species
Human
Available Sizes
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
