Recombinant Human PUMA/BBC3 protein (His Tag)

Product Specifications

Background

Essential mediator of p53/TP53-dependent and p53/TP53-independent apoptosis . Functions by promoting partial unfolding of BCL2L1 and dissociation of BCL2L1 from p53/TP53. Regulates ER stress-induced neuronal apoptosis.

Abbreviation

PUMA; BBC3

Synonyms

BBC 3; BBC3_HUMAN; BCL 2 binding component 3; BCL2 binding component 3; Bbc3; Bcl-2-binding component 3; JFY 1; JFY-1; JFY1; PUMA alpha; PUMA/JFY1; p53 Upregulated Modulator of Apoptosis; p53 up regulated modulator of apoptosis; p53 up-regulated modulator of apoptosis

UniProt

Q9BXH1

Accession Number

Q9BXH1-1

Expression System

E.coli

Tag

N-His

Sequence

Met1-Asn193

Applications

Immunogen

Field of Research

Cell Biology, Signal Transduction, Cancer, Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.