Recombinant Human POSTN Protein (His Tag)

Product Specifications

Background

Periostin (POSTN, PN), originally named as osteoblast-specific factor 2 (OSF-2), is a 90-kDa secreted protein which is now classified as a matricellular protein. It is present in a wide variety of normal adult tissues and fetal tissues, and has a role in bone, tooth and heart development and function. Studies show that periostin is overexpressed in a broad range of human cancer types, includidng lung, ovary, breast and colon cancers. Recent evidence reveals that periostin is expressed by fibroblasts in the normal tissue and in the stroma of the primary tumour, and it is required to allow cancer stem cell maintenance.

Abbreviation

POSTN

Synonyms

Fasciclin-I like; MGC119510; MGC119511; OSF 2; OSF-2; OSF2; Osteoblast specific factor; Osteoblast specific factor 2; Osteoblast specific factor 2 (fasciclin I like) ; Osteoblast-specific factor 2; PDLPOSTN; Periodontal ligament specific periostin; Periostin

UniProt

Q15063

Accession Number

Q15063-1

Expression System

E.coli

Tag

N-His

Sequence

Ala21-Leu365

Field of Research

Signal Transduction, Tags & Cell Markers, Cancer, Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 85% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.