Recombinant Human PIK3CA Protein (His Tag)

Product Specifications

Background

Phosphatidylinositol 3-kinase is composed of an 85 kDa regulatory subunit and a 110 kDa catalytic subunit. The protein encoded by this gene represents the catalytic subunit, which uses ATP to phosphorylate PtdIns, PtdIns4P and PtdIns (4,5) P2. This gene has been found to be oncogenic and has been implicated in cervical cancers. A pseudogene of this gene has been defined on chromosome 22.

Abbreviation

PIK3CA

Synonyms

5-bisphosphate 3-kinase 110 kDa catalytic subunit alpha;5-bisphosphate 3-kinase catalytic subunit alpha isoform; CLOVE; CWS5; MCAP; MCM; MCMTC; MGC142161; MGC142163; Phosphatidy; Phosphatidylinositol 3 kinase catalytic alpha polypeptide; caPI3K; p110 alpha; p110alpha

UniProt

P42336

Accession Number

P42336-1

Expression System

E.coli

Tag

N-His

Sequence

Met1-Pro200

Field of Research

Signal Transduction, Cancer, Immunology

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.