Recombinant Human EGF Protein (Fc Tag)
Product Specifications
Background
This gene encodes a member of the epidermal growth factor superfamily. The encoded protein is synthesized as a large precursor molecule that is proteolytically cleaved to generate the 53-amino acid epidermal growth factor peptide. This protein acts a potent mitogenic factor that plays an important role in the growth, proliferation and differentiation of numerous cell types. This protein acts by binding the high affinity cell surface receptor, epidermal growth factor receptor. Defects in this gene are the cause of hypomagnesemia type 4. Dysregulation of this gene has been associated with the growth and progression of certain cancers. Alternate splicing results in multiple transcript variants
Abbreviation
EGF
Synonyms
Beta urogastrone; beta-urogastrone; EGF; EGF; Epidermal growth factor; HOMG4; OTTHUMP00000219721; OTTHUMP00000219722; Pro epidermal growth factor; URG; Urogastrone
UniProt
P01133
Accession Number
P01133
Expression System
Stable cell line
Tag
C-Fc
Sequence
Asn971-Arg1023
Field of Research
Signal Transduction; Cell Biology; Cancer; Kits; Lysates; Other
Endotoxin
< 1.0 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
30.7 kDa
Observed Molecular Weight
30-40 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
Documents
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