Recombinant Pennsylania firefly Luciferase Protein (His Tag)
Product Specifications
Abbreviation
Pennsylania firefly Luciferase
UniProt
Q27757
Accession Number
Q27757
Expression System
E.coli
Tag
N-His
Sequence
Met1-Leu545
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
59.8 kDa
Observed Molecular Weight
60 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Pennsylania firefly
Available Sizes
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