Recombinant Pennsylania firefly Luciferase Protein (His Tag)

Product Specifications

Abbreviation

Pennsylania firefly Luciferase

UniProt

Q27757

Accession Number

Q27757

Expression System

E.coli

Tag

N-His

Sequence

Met1-Leu545

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Calculated Molecular Weight

59.8 kDa

Observed Molecular Weight

60 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Pennsylania firefly

Available Sizes

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