Recombinant Mouse SH3Y1 Protein (Sumo Tag)
Product Specifications
Background
Differential screening of mouse skin cDNA libraries identified cDNA encoding a protein containing a Src homologous 3 (SH3) domain, a novel protein containing a SH3 domain at the carboxyl terminus, and reporting gene expression patterns during the hair growth cycle. The protein shows significant homology with an the yeast protein YHRO 16c/Ysc 84 in its amino and carboxyl terminus, suggesting that it may be a mouse homolog of the yeast protein. Therefore, we name it the Sh3 domain YSC-like 1, Sh3yl1.
Abbreviation
Sh3yl1
UniProt
O08641
Accession Number
O08641
Expression System
E.coli
Tag
N-Sumo
Sequence
Met1-Asn340
Field of Research
Cell Biology
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
50.3 kDa
Observed Molecular Weight
55 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Mouse
Available Sizes
Documents
Frequently Asked Questions
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