Recombinant Human LC3A/B Protein (Trx Tag)
Product Specifications
Background
LC3A, also known as MAP1LC3A, is one of the light chain subunits that function together with both MAP1A and/or MAP1B. MAP1A and MAP1B are microtubule-associated proteins that mediate the physical interactions between microtubules and components of the cytoskeleton. MAP1A and MAP1B each consist of a heavy chain subunit and multiple light chain subunits. As a light chain subunit, MAP1LC3A has an important part in neuronal development and in maintaining the balance between neuronal plasticity and rigidity. MAP1LC3A is expressed as two alternatively spliced isoforms that are expressed in testis, brain, heart, liver, and skeletal muscle but are absent in thymus and peripheral blood leukocytes.
Abbreviation
LC3A/B
Synonyms
MAP1A/1BLC; MAP1 light chain 3-like protein; MAP1ALC; MAP1LC3B; ATG8F; LC3B; MAP1A/1BLC3; MAP1LC3B-a; microtubule associated protein 1 light chain 3 beta; Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; LC3A/LC3B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B LC3 B; MAP1A/MAP1B light chain 3 B; Microtubule-associated protein 1 light chain 3 beta; MAP1ALC3; Autophagy related protein LC3 A; Autophagy related ubiquitin like modifier LC3 A; Autophagy related ubiquitin like modifier LC3 B; LC3; LC3II; MAP; MAP1 light chain 3 like protein 1; MAP1 light chain 3 like protein 2; MAP1A/1B light chain 3 A; MAP1LC3B a; Microtubule-associated proteins 1A/1B light chain 3B; MLP3B
UniProt
Q9GZQ8
Accession Number
Q9GZQ8
Expression System
E.coli
Tag
N-Trx
Sequence
His27-Val125
Field of Research
Signal Transduction; Neuroscience; Cardiovascular; Cancer; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Product Datasheet
https://789.bio/ea/14zpxd
Product MSDS
https://789.bio/eb/WiiL4R
Calculated Molecular Weight
30.7 kDa
Observed Molecular Weight
31 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
Curated Selection
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