Recombinant Human MPO Protein (Trx Tag)
Product Specifications
Background
Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor , myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.
Abbreviation
MPO
Synonyms
Fj80f; wu:fj80f; MPO;84 kDa myeloperoxidase;89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM; wu:fj80f04;84 kDa myeloperoxidase; Myeloperoxidase;84 kDa myeloperoxidase;89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM; wu:fj80f04
UniProt
P05164
Accession Number
P05164
Expression System
E.coli
Tag
N-Trx
Sequence
Cys606-Ser745
Field of Research
Immunology; Cardiovascular; Cancer; Kits; Lysates; Other; Metabolism
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
35.2 kDa
Observed Molecular Weight
35 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
Documents
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