Recombinant Human MPO Protein (Trx Tag)

Product Specifications

Background

Myeloperoxidase (MPO) is a heme protein synthesized during myeloid differentiation that constitutes the major component of neutrophil azurophilic granules. Produced as a single chain precursor , myeloperoxidase is subsequently cleaved into a light and heavy chain. The mature myeloperoxidase is a tetramer composed of 2 light chains and 2 heavy chains. This enzyme produces hypohalous acids central to the microbicidal activity of neutrophils.

Abbreviation

MPO

Synonyms

Fj80f; wu:fj80f; MPO;84 kDa myeloperoxidase;89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM; wu:fj80f04;84 kDa myeloperoxidase; Myeloperoxidase;84 kDa myeloperoxidase;89 kDa myeloperoxidase; EC 1.11.1.7; EC1.11.2.2; fj80f04; MPO; mpx; myeloid-specific peroxidase; Myeloperoxidase; Myeloperoxidase heavy chain; Myeloperoxidase light chain; PERM; wu:fj80f04

UniProt

P05164

Accession Number

P05164

Expression System

E.coli

Tag

N-Trx

Sequence

Cys606-Ser745

Field of Research

Immunology; Cardiovascular; Cancer; Kits; Lysates; Other; Metabolism

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.