Recombinant Human PFN1 Protein (Sumo Tag)
Product Specifications
Background
This gene encodes a member of the profilin family of small actin-binding proteins. The encoded protein plays an important role in actin dynamics by regulating actin polymerization in response to extracellular signals. Deletion of this gene is associated with an Miller-Dieker syndrome, and the encoded protein may also play a role in Huntington disease. Multiple pseudogenes of this gene are located on chromosome 1.
Abbreviation
PFN1
Synonyms
Actin binding protein; ALS18; Epididymis tissue protein Li 184a; OTTHUMP00000125244; PFN 1; Pfn; PFN1; PROF1; Profilin I; Profilin-1; Profilin1; ProfilinI
UniProt
P07737
Accession Number
P07737
Expression System
E.coli
Tag
N-Sumo
Sequence
Met1-Tyr140
Field of Research
Cancer; Cell Biology; Neuroscience; Signal Transduction; Tags and Cell Markers
Endotoxin
< 10 EU/mg of the protein as determined by the LAL method.
Purity
> 90% as determined by reducing SDS-PAGE.
Bioactivity
Not validated for activity
Reconstitution
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
Shipping Conditions
This product is provided as lyophilized powder which is shipped with ice packs.
Storage Conditions
Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
Calculated Molecular Weight
28.3 kDa
Observed Molecular Weight
32 kDa
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.
Species
Human
Available Sizes
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