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Recombinant Human BMP-2 Protein (Trx Tag)

Product Specifications

Background

Bone morphogenetic protein-2 (BMP-2) is a member of the transforming growth factor-beta (TGFB) superfamily. BMP2 is synthesized as a 60 kDa precursor that is processed in the secretory pathway to a small 18 kDa monomer, 2 monomers then associate to form the active 30 kDa homodimer, which binds to its receptor. There is also a 40-45 kDa form of BMP2, as an amino-terminal propeptide. BMP2 can induce bone formation and regeneration during early embryonic development. It is involved in the hedgehog pathway, TGF beta signaling pathway, and cytokine-cytokine receptor interaction.

Abbreviation

BMP-2

Synonyms

BDA2; BMP-2; BMP-2A; Bmp2; BMP2; BMP2A; Bone morphogenetic protein 2; Bone morphogenetic protein 2A

UniProt

P12643

Accession Number

P12643

Expression System

E.coli

Tag

N-Trx

Sequence

Gln283-Arg396

Field of Research

Signal Transduction; Stem Cells; Cancer; Cardiovascular

Endotoxin

< 10 EU/mg of the protein as determined by the LAL method.

Purity

> 90% as determined by reducing SDS-PAGE.

Bioactivity

Not validated for activity

Reconstitution

It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Shipping Conditions

This product is provided as lyophilized powder which is shipped with ice packs.

Storage Conditions

Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Product Datasheet

https://789.bio/ea/y54pyh

Product MSDS

https://789.bio/eb/O8pWD0

Calculated Molecular Weight

32.4 kDa

Observed Molecular Weight

32 kDa

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose and 5% Mannitol.

Species

Human

Available Sizes

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