CheKine™ Micro Soil Alkaline Xylanase (S-BAX) Activity Assay Kit

Xylanase (EC3.2.1.8) is mainly produced by microorganisms, which can catalyze the hydrolysis of xylan, also known as pentosanase or hemicellulase, which can decompose the cell wall and β -glucan of raw materials in brewing or feed industry, reduce the viscosity of materials in brewing, promote the release of effective substances, reduce non-starch polysaccharides in feed and promote the absorption and utilization of nutrients, so it is widely used in brewing and feed industry, and BAX is generally isolated from the optimal growth. CheKine™ Micro Soil Alkaline Xylanase (S-BAX) Activity Assay Kit can detect biological samples such as soli. In this kit, S-BAX catalyzes the degradation of xylan into reducing oligosaccharides and monosaccharides in alkaline environment, and further reacts with 3,5- dinitrosalicylic acid in boiling water bath, with a characteristic absorption peak at 540 nm. The color of the reaction solution is proportional to the amount of reducing sugar produced by enzymolysis. By measuring the increase rate of absorption value of the reaction solution at 540 nm, the activity of S-BAX can be calculated.

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Storage at 4°C and Keep from light immediately upon receipt. Kit has a storage time of 6 months from receipt. Refer to list of materials supplied for storage conditions of individual components.

• If not assayed immediately, samples can be stored at -80°C for one month.• It is recommended to perform several dilutions of your sample to ensure the readings are within the standard value range.• Fresh samples are necessary for good results. If don't perform the assay at the same time, it's better to complete the Sample Preparation step before storing the samples.