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Recombinant Phospho-cdc2 (Tyr15) Monoclonal Antibody

Product Specifications

Background

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Abbreviation

CDK1

UniProt

P06493

Host

Rabbit

Reactivity

Human

Immunogen

A synthetic phosphopeptide corresponding to residues around Tyr15 of the Human cdc2

Target

CDC; CDK; CDKN; cyclin-dependent kinase; P34CDC; CDK1; CDC2; CDC28A; P34CDC2; cyclin-dependent kinase 1; CDKN1; CDC2; CDC28A; cyclin-dependent kinase 1; P34CDC2; cyclin dependent kinase 1874

Clonality

Monoclonal

Clone

5B3

Conjugation

Unconjugated

Applications

WB

Field of Research

Cell Biology; Epigenetics and Nuclear Signaling; Cancer; Neuroscience; Metabolism

Purification

Protein A

Concentration

1 mg/mL

Dilution

WB 1:5000-1:50000

Buffer

10 mM sodium HEPES, 150 mM NaCl, 100 μg/mL protein protectant, 50% glycerol, pH 7.5

Shipping Conditions

Ice bag

Storage Conditions

This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Calculated Molecular Weight

34 kDa

Observed Molecular Weight

34 kDa

Isotype

IgG

Available Sizes

Curated Selection

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