Anti-KOS1 Antibody

Rabbit Polyclonal Antibody specific to Kos1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 2.7.10.2, Kinase of embryonic stem cells

Gene Name

Tnk1

NCBI Gene ID

UniProt

Q99ML2

Cellular Locus

Membrane, Cytoplasm

Host

Rabbit

Reactivity

Mouse

Immunogen

Synthetic peptide corresponding to amino acids 421-434 (QNQVQSLPAGSAEP) of mouse Kos1 conjugated to KLH at the N-terminus.

Target Antigen

Non-receptor tyrosine-protein kinase TNK1

Target

KOS1

Clonality

Polyclonal

Type

Antibody

Applications

WB, ELISA

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

May function in signaling pathways utilized broadly during fetal development and more selectively in adult tissues and in cells of the lymphohematopoietic system. Could specifically be involved in phospholipid signal transduction (By similarity). Involved in negative regulation of cell growth. Has tumor suppressor properties. Plays a negative regulatory role in the Ras-MAPK pathway. {PubMed:12789265, PubMed:18974114}.

Additionnal Information

Immunoblotting: use at 1:500 dilution. A band of approximately 47 kDa is detected. <br><br>Positive control: transfected cell lysate.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes mouse Kos1 protein.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Kinase of embryonic stem cells (Kos1) is a non-receptor protein tyrosine kinase (NRPTK) and a new member of the Ack family of NRPTKs. Kos1 is a ubiquitously expressed 47-kDa protein with autotyrosine kinase activity that is developmentally regulated during embryogenesis. Kos1 is also upregulated following IL3 withdrawal from factor-dependent murine NSF/N1.H7 cells that undergo apoptosis, suggesting a role in growth inhibition. Data support a negative regulatory role for Kos1 in regulating the Ras-Raf1-MAPK growth pathway by a mechanism that requires its autotyrosine kinase activity.