Phosphate Buffer, 10X
This buffer is supplied as 10X solution. It is used as 1X in all western blotting, ELISA and Chemiluminescence assays as a washing buffer, making blocking solution, diluting HRP-secondary and primary antibody etc.
Product Specifications
Applications
WB, IHC, ELISA
Assay Principle
Preparation: Dilute this 10X buffer 10 times (e.g. 90 ml of deionized or distilled water + 10 ml of this buffer), mix well, and store 1 X buffer solution at room temperature or 2-8oC for several weeks.
Application: Wash membrane or ELISA plates for 10 minutes each for three times.. For 10x10 cm membrane use at least 50 ml buffer with shaking gently. This buffer is used in between steps in immunoassays protocol. For diluting blocking solution, primary antibody or HRP-secondary antibody, please refer to WB or ELISA protocol.
Shipping Conditions
Ambient Temperature
Storage Conditions
Room Temperature
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
PPT-10104 100 µg
Clostridium perfringens plc/Phospholipase C/Alpha-toxin Recombinant Protein
Ask
View Details 
MBS6136302-01 0.1 mL
DR3 (Death Receptor 3, DR-3, Apo-3, Apoptosis-inducing Receptor AIR, TRAMP, Death Domain Receptor 3, DDR3, Death Receptor beta, Lymphocyte Associated Receptor of Death, LARD, TR3, Translocating Chain Association Membrane Protein, Tumor Necrosis Factor Rec
Ask
View Details MBS6136302-02 5x 0.1 mL
DR3 (Death Receptor 3, DR-3, Apo-3, Apoptosis-inducing Receptor AIR, TRAMP, Death Domain Receptor 3, DDR3, Death Receptor beta, Lymphocyte Associated Receptor of Death, LARD, TR3, Translocating Chain Association Membrane Protein, Tumor Necrosis Factor Rec
Ask
View Details 