Anti-TASK1 Potassium Channel Antibody

Mouse Monoclonal Antibody specific to TASK1 Potassium Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Acid-sensitive potassium channel protein TASK-1, TWIK-related acid-sensitive K(+ channel 1, Two pore potassium channel KT3.1, Two pore K(+ channel KT3.1

Gene Name

Kcnk3

NCBI Gene ID

UniProt

O54912

Accession Number

NP_203694.1

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 251-411 (cytoplasmic C-terminus) of rat TASK1. This sequence is 96

Target Antigen

Potassium channel subfamily K member 3

Target

TASK1 Potassium Channel

Clonality

Monoclonal

Isotype

IgG2b

Type

Antibody

Applications

WB, IHC, ICC/IF

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

pH-dependent, voltage-insensitive, background potassium channel protein. Rectification direction results from potassium ion concentration on either side of the membrane. Acts as an outward rectifier when external potassium concentration is low. When external potassium concentration is high, current is inward. {UniProtKB:O14649}.

Additionnal Information

Immunoblotting: use at 1-5ug/mL. A band of ~50kDa is detected. Immunofluorescence: use at 10ug/mL. These are recommended concentrations. Endusers should determine optimal concentrations for their application.

Storage Conditions

This product is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat TASK1.

Formulation

PBS, pH 7.4, 0.1% sodium azide, 50% glycerol.

Buffer pH

pH 7.4

Target Background

TASK1 channels are members of the two-pore domain family of potassium channels whose structure consists of two pore-forming regions flanked by four membrane-spanning domains. The activity of these channels is sensitive to changes in extracellular pH in the physiological range. Like other two-pore domain family members, these channels show little time or voltage dependence. Thus they have characteristics of leak K+ channels, generating background currents that contribute to membrane potential and the shaping of cell excitability.