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Myc tag, AlpHcAbs® Mouse IgG2b

Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody is designed for detecting Myc tag fusion protein specifically. Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody is recombinant fab of alpaca IgG1 fused to mouse IgG2b Fc. Based on western blot and ELISA, Anti-Myc tag, AlpHcAbs® Mouse IgG2b antibody reacts with the Myc tag sequence (EQKLISEEDL) selectively, no reactivity with other proteins.

Product Specifications

Host

Alpaca

Species Reactivity

Universal

Immunogen

Myc tag fusion protein

Isotype

IgG2b

Conjugation

Non-conjugated

Type

Alpaca monoclonal Antibody

Applications

WB, ICC/IF, ELISA, IP, FC

Concentration

1 mg/mL

Dilution

WB: 1:10,000-1:50,000 ;ICC/IF: 1:200-1:1000 ;ELISA: 1:10,000-1:50,000 ;IP: 1-2ug/sample ;FC: 1µg for 106 cells ;Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

Purity

Recombinant Expression and Affinity purified

Form

Liquid

Buffer

Liquid, 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose, 0.01% proclin300, 50% Glycerol

Storage Conditions

Store at –20°C, (Avoid freeze / thaw cycles), Stable for 12 months at -20°C

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