Anti-Hu CD74 PE

CD74 (the MHC II-associated invariant chain, Ii) is a type II transmembrane protein expressed in antigen-presenting cells, that serves as MHC II chaperone which promotes MHC II trafficking from the ER to endocytic compartments, prevents peptide binding in the ER and contributes to peptide editing in the MHC II compartment; it is also an accessory signaling molecule implicated e.g. in malignant B cell proliferation. Stimulation of cell surface CD74 leads to NFkappaB activation, which enables entry of the stimulated cell into the S phase. CD74 binds pro-inflammatory cytokine MIF with high affinity and interacts with CD44. Binding of Vpu, an HIV1 protein, to CD74 modulates MHC II presentation.

Product Specifications

Certification

RUO

Immunogen

SU-DHL-4 lymphoma cells

Target Antigen

CD74

Clone

LN2

Applications

FC (QC tested)

Format

Buffer

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

References & Citations

*Burton JD, Ely S, Reddy PK, Stein R, Gold DV, Cardillo TM, Goldenberg DM: CD74 is expressed by multiple myeloma and is a promising target for therapy. Clin Cancer Res. 2004 Oct 1;10 (19) :6606-11., URL: http://www.ncbi.nlm.nih.gov/pubmed/15475450, *Badve S, Deshpande C, Hua Z, Lögdberg L: Expression of invariant chain (CD 74) and major histocompatibility complex (MHC) class II antigens in the human fetus. J Histochem Cytochem. 2002 Apr;50 (4) :473-82., URL: http://www.ncbi.nlm.nih.gov/pubmed/11897800 , *Leng L, Metz CN, Fang Y, Xu J, Donnelly S, Baugh J, Delohery T, Chen Y, Mitchell RA, Bucala R: MIF signal transduction initiated by binding to CD74. J Exp Med. 2003 Jun 2;197 (11) :1467-76., URL: http://www.ncbi.nlm.nih.gov/pubmed/12782713 , *Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989) ., URL: , *Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995) ., URL: , *Perez MT, Cabello-Inchausti B, Castellano-Sanchez A, Kottiech S, Davila E, Willis I, Barkin J: Primary gastroesophageal-ileal hodgkin lymphoma. Arch Pathol Lab Med. 2002 Dec;126 (12) :1534-7., URL: https://pubmed.ncbi.nlm.nih.gov/12456218/, *Freeman GJ, Cardoso AA, Boussiotis VA, Anumanthan A, Groves RW, Kupper TS, Clark EA, Nadler LM: The BB1 monoclonal antibody recognizes both cell surface CD74 (MHC class II-associated invariant chain) as well as B7-1 (CD80), resolving the question regarding a third CD28/CTLA-4 counterreceptor. J Immunol. 1998 Sep 15;161 (6) :2708-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/9743327

Other References

*Hussain A, Wesley C, Khalid M, Chaudhry A, Jameel S: Human immunodeficiency virus type 1 Vpu protein interacts with CD74 and modulates major histocompatibility complex class II presentation. J Virol. 2008 Jan;82 (2) :893-902. , URL: https://www.ncbi.nlm.nih.gov/pubmed/17959659?ordin, *Gore Y, Starlets D, Maharshak N, Becker-Herman S, Kaneyuki U, Leng L, Bucala R, Shachar I: Macrophage migration inhibitory factor induces B cell survival by activation of a CD74-CD44 receptor complex. J Biol Chem. 2008 Feb 1;283 (5) :2784-92., URL: https://www.ncbi.nlm.nih.gov/pubmed/18056708?ordin, *Lantner F, Starlets D, Gore Y, Flaishon L, Yamit-Hezi A, Dikstein R, Leng L, Bucala R, Machluf Y, Oren M, Shachar I: CD74 induces TAp63 expression leading to B-cell survival. Blood. 2007 Dec 15;110 (13) :4303-11., URL: https://www.ncbi.nlm.nih.gov/pubmed/17846227?ordin, *Stein R, Mattes MJ, Cardillo TM, Hansen HJ, Chang CH, Burton J, Govindan S, Goldenberg DM: CD74: a new candidate target for the immunotherapy of B-cell neoplasms. Clin Cancer Res. 2007 Sep 15;13 (18 Pt 2) :5556s-5563s., URL: https://www.ncbi.nlm.nih.gov/pubmed/17875789?ordin, *Starlets D, Gore Y, Binsky I, Haran M, Harpaz N, Shvidel L, Becker-Herman S, Berrebi A, Shachar I: Cell-surface CD74 initiates a signaling cascade leading to cell proliferation and survival. Blood. 2006 Jun 15;107 (12) :4807-16., URL: https://www.ncbi.nlm.nih.gov/pubmed/16484589?ordin

Storage Conditions

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Specificity

The antibody LN2 recognizes an extracellular epitope of CD74 (the MHC II-associated invariant chain, Ii), a type II transmembrane protein expressed in antigen-presenting cells, as well as e.g. on activated neoplastic cells in T cell lymphomas, in lymph node germinal center, mantle zone B cells, histiocytes, interdigitating reticulum cells, Langerhans cells, thymic dendritic cells and peripheral blood B lymphocytes.