Human HLA-A*03:01&B2M Monomer Protein (Peptide free, MALS verified)
Human HLA-A*03:01&B2M Monomer Protein (HLM-H52H3) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M) (Accession # P04439 (HLA-A*03:01) & P61769 (B2M) ) .
Product Specifications
Background
Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa and 15.7 kDa. The protein migrates as 42-45 kDa and 16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
P04439 (HLA-A*03:01) & P61769 (B2M)
Expression Region
Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M)
Host
HEK293
Target
HLA-A*0301 & B2M
Conjugation
Unconjugated
Tag
C-10xHis
Source
Human
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized Human HLA-A*03:01&B2M Monomer Protein (Cat. No. HLM-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
37.7 kDa and 15.7 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
200ug*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/HLM-H52H3.pdf
Species
Human
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