Human HLA-A*03:01&B2M Monomer Protein (Peptide free, MALS verified)

Major histocompatibility complex (MHC), group of genes that code for proteins found on the surfaces of cells that help the immune system recognize foreign substances. MHC proteins are found in all higher vertebrates. In human beings the complex is also called the human leukocyte antigen (HLA) system.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 37.7 kDa and 15.7 kDa. The protein migrates as 42-45 kDa and 16 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

P04439 (HLA-A*03:01) & P61769 (B2M)

Expression Region

Gly 25 - Thr 305 (HLA-A*03:01) & Ile 21 - Met 119 (B2M)

Host

HEK293

Target

HLA-A*0301 & B2M

Conjugation

Unconjugated

Tag

C-10xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

1.0 EU per μg

Purity

90%

Bioactivity

Immobilized Human HLA-A*03:01&B2M Monomer Protein (Cat. No. HLM-H52H3) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-2 ng/mL (QC tested).