Anti-GABA(B)R1 Antibody

Mouse Monoclonal Antibody specific to GABA(B)R1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

Gene Name

Gabbr1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=Gabbr1">Gabbr1</a>

UniProt

Q9Z0U4

Cellular Locus

Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell projection, dendrite, Perikaryon

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein corresponding to aa 873-977 (cytoplasmic C-terminus) of rat GABA(B)R1 (accession no. NP_112290.2).

Target Antigen

γ-aminobutyric acid type B receptor subunit 1

Target

GABA(B)R1

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ICC/IF

Field of Research

Neuroscience

Purification Method

Purified by Protein G affinity chromatography

Concentration

1.0 mg/mL

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872315, PubMed:9872317, PubMed:9872744). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:9872317, PubMed:10658574). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10075644, PubMed:9872315, PubMed:9872744, PubMed:10924501). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:9069281, PubMed:10457184, PubMed:9872315, PubMed:9872744, PubMed:10924501, PubMed:10692480). Calcium is required for high affinity binding to GABA (PubMed:10692480). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9872744). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9872744, PubMed:10924501, PubMed:10692480). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (By similarity). {UniProtKB:Q9UBS5, PubMed:10075644, PubMed:10457184, PubMed:10658574, PubMed:10692480, PubMed:10924501, PubMed:9069281, PubMed:9872315, PubMed:9872744}.

Additionnal Information

Immunoblotting: use at 1-2ug/mL. A band of ~115kDa is detected. Immunocytochemistry: use at 1-5ug/mL. These are recommended concentrations. Enduser should determine optimal concentrations for their applications. Positive control: Rat brain membranes.

Storage Conditions

This product is stable for at least 1 year at -20°C. Freeze in multiple aliquots to avoid repeated freeze-thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat GABA(B)R1. It does not cross- react with GABA(B)R2.

Formulation

PBS, pH 7.4, 50% glycerol, 0.09% sodium azide.Purified by Protein G affinity chromatography.

Buffer pH

pH 7.4

Target Background

GABA (g-aminobutyric acid) is the primary inhibitory neurotransmitter in the central nervous system and interacts with three different receptors: GABA(A), GABA(B), and GABA(C). GABA(B) receptor is coupled to G proteins that modulate slow inhibitory synaptic transmission. Functional GABA(B) receptors form heterodimers of GABA(B)R1 and GABA(B)R2 in which GABA(B)R1 binds a ligand and GABA(B)R2 is the primary G protein contact site.