Anti-Ku80 Antibody

Rabbit Polyclonal Antibody specific to Ku80

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

EC 3.6.4.-, 86 kDa subunit of Ku antigen, ATP-dependent DNA helicase 2 subunit 2, ATP-dependent DNA helicase II 80 kDa subunit, CTC box-binding factor 85 kDa subunit, CTC85, CTCBF, DNA repair protein XRCC5, Ku80, Ku86, Lupus Ku autoantigen protein p86, Nuclear factor IV, Thyroid-lupus autoantigen, TLAA, X-ray repair complementing defective repair in Chinese hamster cells 5

Gene Name

XRCC5

NCBI Gene ID

UniProt

P13010

Accession Number

NP_066964

Cellular Locus

Nucleus, Nucleus, nucleolus, Chromosome

Host

Rabbit

Reactivity

Human, Mouse, Rat, Hamster

Immunogen

Synthetic peptide corresponding to amino acids 323-338 (FSKVDEEQMKYKSEGK) of Ku80 conjugated to KLH at the C-terminus.

Target Antigen

X-ray repair cross-complementing protein 5

Target

Ku80

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

DNA

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Single-stranded DNA-dependent ATP-dependent helicase that plays a key role in DNA non-homologous end joining (NHEJ) by recruiting DNA-PK to DNA (PubMed:7957065, PubMed:8621488, PubMed:12145306, PubMed:11493912). Required for double-strand break repair and V(D)J rPubMed:11493912, PubMed:12145306, PubMed:20383123, PubMed:28712728, PubMed:32103174, PubMed:7957065, PubMed:8621488}.

Additionnal Information

Immunoblotting: use at 1:500 dilution. A band of approximately 80 kDa is detected. <br><br>Positive control: A431 and AR42J whole cell lysates.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human, mouse, rat, and hamster Ku80.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Telomere length maintenance, essential to chromosome stability and genome integrity, is regulated by telomerase and telomere-associated factors. The Ku proteins are eukaryotic nuclear proteins involved in DNA repair and telomere maintenance. They exist as a heterodimer of 70 and 80 kDa subunits (Ku 70 and Ku 80). Ku heterodimers bind to DNA ends at telomeres and at double-strand breaks where they interact with DNA-dependent protein kinase (DNA PK) and participate in targeting of DNA PK to DNA. The Ku proteins may participate in DNA repair and replication, cell signaling, proliferation, transcriptional activation, and apoptosis.