Human Mesothelin / MSLN (296-580) Protein, His Tag, premium grade

Mesothelin (MSLN) is also known as CAK1 antigen, Pre-pro-megakaryocyte-potentiating factor, which belongs to the mesothelin family. Mesothelin / MSLN can be proteolytically cleaved into the following two chains by a furin-like convertase: Megakaryocyte-potentiating factor (MPF) and the cleaved form of mesothelin. Both MPF and the cleaved form of mesothelin are N-glycosylated. Mesothelin / MSLN can interacts with MUC16. The membrane-anchored forms of MSLN may play a role in cellular adhesion. MPF potentiates megakaryocyte colony formation in vitro.

Product Specifications

Background

Specifications

This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 33.0 kDa. The protein migrates as 38-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Accession Number

AAH09272

Expression Region

Glu 296 - Gly 580

Host

HEK293

Target

Mesothelin

Conjugation

Unconjugated

Tag

C-6xHis

Source

Human

Stability

-20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Endotoxin

0.01 EU per μg

Purity

90%

Bioactivity

Immobilized Human Mesothelin (296-580), His Tag, premium grade (Cat. No. MSN-H522a) at 0.5 μg/mL (100 μL/well) can bind Anti-MSLN MAb, Human IgG1 with a linear range of 0.2-3 ng/mL (QC tested).