QuicKey Pro Human PIIINP (N-Terminal Procollagen III Propeptide) ELISA Kit

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PIIINP. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human PIIINP are added to the micro ELISA plate wells. Human PIIINP in samples (or standards) combines with the coated antibody and HRP linked detection antibody special to Human PIIINP.Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PIIINP. The concentration of Human PIIINP in the samples is then determined by comparing the OD of the samples to the standard curve.