SARS-CoV-2 Spike S2 Protein, His Tag
SARS-CoV-2 Spike S2, His Tag (S2N-C52H5) is expressed from human 293 cells (HEK293) . It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1) . Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) are introduced to prevent the formation of aggregates in the course of protein production.
Product Specifications
Background
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
Specifications
This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 60.0 kDa. The protein migrates as 65-115 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Accession Number
QHD43416.1
Expression Region
Ser 686 - Pro 1213
Host
HEK293
Target
Spike S2
Conjugation
Unconjugated
Tag
C-10xHis
Source
SARS-CoV-2
Stability
● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Endotoxin
1.0 EU per μg
Purity
90%
Bioactivity
Immobilized SARS-CoV-2 Spike S2, His Tag (Cat. No. S2N-C52H5) at 1 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S2 Antibody, Human IgG1 with a linear range of 0.4-3 ng/mL (QC tested).
Format
Powder
Buffer
PBS, pH7.4
Additives
Trehalose
Molecular Weight
60.0 kDa
Shipping Conditions
RT
Storage Conditions
-20°C
Package Size
1mg*1
Product Datasheet
Https://www.acrobiosystems.com/static/main/products/pdf/ds/S2N-C52H5.pdf
Species
SARS-CoV-2
Curated Selection
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