NDUFA1 Rabbit pAb

The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH: ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008],

Product Specifications

Background

The human NDUFA1 gene codes for an essential component of complex I of the respiratory chain, which transfers electrons from NADH to ubiquinone. It has been noted that the N-terminal hydrophobic domain has the potential to be folded into an alpha-helix spanning the inner mitochondrial membrane with a C-terminal hydrophilic domain interacting with globular subunits of complex I. The highly conserved two-domain structure suggests that this feature is critical for the protein function and might act as an anchor for the NADH:ubiquinone oxidoreductase complex at the inner mitochondrial membrane. However, the NDUFA1 peptide is one of about 31 components of the "hydrophobic protein" (HP) fraction of complex I which is involved in proton translocation. Thus the NDUFA1 peptide may also participate in that function. [provided by RefSeq, Jul 2008]

Synonyms

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (Complex I-MWFE;CI-MWFE;NADH-ubiquinone oxidoreductase MWFE subunit)

Gene ID

4694

UniProt

O15239

Cellular Locus

Mitochondrion inner membrane ; Single-pass membrane protein ; Matrix side.

Host

Rabbit

Species Reactivity

Human,Mouse,Rat

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human NDUFA1 AA range: 20-100

Clonality

Polyclonal

Isotype

IgG

Source

Rabbit

Applications

IHC, ELISA

Validated Applications

IHC,ELISA

Stability

-20°C/1 year

Concentration

1 mg/mL

Dilution

IHC-p 1:50-200, ELISA (peptide) 1:5000-20000

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Product Datasheet

https://www.elkbiotech.com/upload/file/Antibodies/pAb/ES14533-1.pdf

Subcellular Location

Mitochondrion inner membrane ; Single-pass membrane protein ; Matrix side .

Other Product Names

NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 1 (Complex I-MWFE; CI-MWFE; NADH-ubiquinone oxidoreductase MWFE subunit)

Gene ID (Human)