EliKine™ Mouse GLP-1 Competitive ELISA Kit

GLP-1 is a peptide hormone released from intestinal L-cells upon nutrient consumption. It bindsthe GLP-1 receptorin the pancreas and displays various antidiabetic effects by potentiating glucose-induced secretion of insulin from pancreatic β-cells, increasing insulin expression. EliKine™ Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1 (GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

EliKine™ Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1 (GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value.

• Do not mix components from different kit lots or use reagents beyond the kit expiration date.• Allow all reagents to warm to room temperature for at least 30 minutes before opening.• Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.• Unused wells must be kept desiccated at 4 °C in the sealed bag provided.• Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.• It is recommended that all samples and standards be assayed in duplicate or triplicate.