Anti-HCN4 Cyclic nucleotide-gated channel Antibody

Mouse Monoclonal Antibody specific to HCN4 Cyclic nucleotide-gated Channel

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

Anti-HCN4 Cyclic nucleotide-gated Channel Mouse Monoclonal Antibody

Gene Name

Hcn4

NCBI Gene ID

UniProt

Q9JKA7

Accession Number

NP_067690.1

Cellular Locus

Cell membrane, Multi-pass membrane protein

Host

Mouse

Reactivity

Human, Mouse, Rat

Immunogen

Fusion protein aa 1019-1108 (cytoplasmic C-terminus) of rat HCN4 (accession no. Q9JKA7).

Target Antigen

Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 4

Target

HCN4 Cyclic nucleotide-gated channel

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, IHC, ICC/IF, AM, FACS

Field of Research

Ion Channels

Purification Method

Purified by Protein G affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Hyperpolarization-activated ion channel with very slow activation and inactivation exhibiting weak selectivity for potassium over sodium ions. May contribute to the native pacemaker currents in heart (If) that regulate the rhythm of heart beat. May contribute to the native pacemaker currents in neurons (Ih) (By similarity). May mediate responses to sour stimuli. {PubMed:11675786}.

Additionnal Information

Immunoblotting: use at 1-10ug/mL. A band of ~1300kDa is detected.Immunohistochemistry and Immunocytochemistry: use at 0.1-1ug/mL Immunofluorescence: use at 1-10ug/mL These are recommended concentrations. User should determine optimal concentrations for their application. Positive control: COS cell lysate transiently transfected with HCN4.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C.

Specificity

This antibody recognizes human, mouse, and rat HCN4. It does not cross-react with other HCNs.

Formulation

PBS, pH 7.4; 50% glycerol, 0.09% sodium azide.

Buffer pH

pH 7.4

Target Background

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient. Hyperpolarization-activated cation channels of the HCN gene family, such as HCN4, play a crucial role in the regulation of cell excitability. They contribute to spontaneous rhythmic activity in the heart and brain.