Mouse anti Human CD5 FITC - CD20 PE

Identification of Human helper/inducer T cells expressing the 67,000 M.W. surface antigen, 85% peripheral blood lyymphocytes that form rosettes with sheep red blood cells (E+), and a small subset of B cells. Identicication of CD20 PE Human B cells associated approximately 10% of peripheral blood lymphocytes.

PBMC: Add 10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8ºC. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20ºC). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.

This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

1. Anti-DNA Antibody Production by CD5+ and CD5- B Cells of Patients with Systemic Lupus Erythematosus. Suzuki N., Sakane T., Engleman EG, J. Clin. Invest. 1990 Ja;85(1):238-47. 2. Characteristics of CD11c+ CD5+ Chronic B cell Leukemias and the Identification of Novel Peripheral Blood B cell Subsets with Chronic Lymphoid Leukemia Immunophenotypes. Wormsley SB, Baird SM, Gadol N., Rai KR, Sobol RE, Blood 1990 July. 76(1):123-30. 3. Stimulation of CD5 Enhances Signal Transduction by the T cell Antigen Receptor. Imboden JB, June CH, McCutcheon MA, Ledbetter JA, J. Clin. Invest. 1990 Ja;85(1):130-4. 4. Surface Immunoglobulin Ligands and Cytokines Differentially Affect Proliferation and Antibody Production by Human CD5+ and CD5- B Lymphocytes. Nawata Y., Stall Am, Herzenberg LA, Eugui Em, Allison AC, Int. Immunol. 1990;2(7):603-14. 5. Evidence for Differential Responsiveness of Human CD5+ and CD5- B cell Subsets to T cell Independent Mitogens. Zupo S., Dono M., Azzoni L., Chiorazzi N., Ferrarini M., Eur. J. Immunol. 1991 Fe. 21(2):351-9. 6. In Vivo and In Vitro Expression of Myeloid Antigens on B-lineage Acute Lymphoblastic Leukemia Cells. Leukemia, 1991, Ja;5(1):19-25. Hara, J.; Kawa-Ha, K.; Yumura-Yagi, K.; Kurahashi, H.; Tawa, A.; Ishihara, S.; Inoue, M.; Murayama, N.; Okada, S. 7. Activation of Dense Human Tonsilar B Cells. Induction of C-Myc Gene Expression via Two Distinct Signal Transduction Pathways. Immunol., 1991, Feb;146(3):846-53. White, M. W.; McConnnell, F.; Shu, G.L.; Morris, D.R.; Clark, E.A. 8. Differential Effects of Low and High Concentrations of Interleukin 6 on Human B Cell. Eur J. Immunol. 1990 No. 20(11) :2389-93. Levy, Y.; Fermand, J.P; Broute, J.C. 9. Immunophenotypes in Classical B-cell Chronic Lymphocytic Leukemia. Correlation with Normal Cellular Counterpart and Clinical Findings. Cancer, 1990, Oct. 1;66(8):1738-42. Baldini, L.; Cro, L.; Cortelezzi, A.; Calori, R.; Nobili, L.; Maiolo, A.T.; Polli, E. E.