Anti-GABABR1 Antibody

Rabbit Polyclonal Antibody specific to GABA(B)R1

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

GABA-B receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1

Gene Name

GABBR1

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=GABBR1">GABBR1</a>

UniProt

Q9UBS5

Cellular Locus

Cell membrane, Cell junction, synapse, postsynaptic cell membrane, Cell projection, dendrite

Host

Rabbit

Reactivity

Human

Immunogen

Peptide corresponding to aa 33-54 of the GABABR1 subunit.

Target Antigen

γ-aminobutyric acid type B receptor subunit 1

Target

GABABR1

Clonality

Polyclonal

Type

Antibody

Applications

Field of Research

Neuroscience

Purification Method

Purified by Protein A affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2 (PubMed:9872316, PubMed:9872744, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054). Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins (PubMed:18165688). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase (PubMed:10906333, PubMed:10773016, PubMed:10075644, PubMed:9872744, PubMed:24305054). Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis (PubMed:10075644). Calcium is required for high affinity binding to GABA (By similarity). Plays a critical role in the fine-tuning of inhibitory synaptic transmission (PubMed:9844003). Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials (PubMed:9844003, PubMed:9872316, PubMed:10075644, PubMed:9872744, PubMed:22660477). Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen (PubMed:9844003, PubMed:9872316, PubMed:24305054). {UniProtKB:Q9Z0U4, PubMed:10075644, PubMed:10773016, PubMed:10906333, PubMed:15617512, PubMed:18165688, PubMed:22660477, PubMed:24305054, PubMed:9844003, PubMed:9872316, PubMed:9872744, ECO:0000305}.; Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. In immunoblots, a band of approximately 120 kD is detected.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes the GABABR1 subunit. The GABAB receptor is the first discovered G-protein-coupled receptor whose function requires two subunits, R1 and R2, to form a functional receptor. The R1 subunit extracellular domain binds GABA, and the R2 subunit functions in G-protein activation.