Beta-2 Microglobulin (Human) ELISA Kit

Beta-2 microglobulin (B2M) is a low MW protein (11.8 kDa) that is a component of major histocompatibility complex (MHC) Class I molecule. It is expressed on the surface of all nucleated cells (excluding red blood cells). One of the functions of this protein is to present antigens to cytotoxic T-cells and NK cells. It is produced at a constant rate and is filtered from the blood by the kidneys. Excess concentration of B2M in serum correlates with several pathological conditions such as autoimmune disease, renal disease, inflammation, and cancer. BioVision’s Beta-2 Microglobulin (Human) ELISA kit is designed to quantitatively measure the amount of B2M in human serum, plasma, and other biological fluids. The kit is based on the Sandwich ELISA principle. Test samples, Standards, and Biotinylated Detection antibody are added to the wells pre-coated with capture antibody and subsequently washed with Wash Buffer. The HRP-Streptavidin is added and any unattached conjugates are washed off with Wash Buffer. The HRP enzymatic reaction is detected by the addition of TMB-substrate. Finally, the reaction is terminated with an acidic stop solution. The color developed is proportional to the amount of B2M in the sample or standard.