ProtoBlot Rapid Western Transfer Buffer

Description Catalog number:EC-878Size: 1 LiterBlot in half the time versus conventional electroblotting buffersVery high-efficiency transferOptimal pH; less harsh on proteinsProtoBlot Rapid Western Blotting Buffer is a relatively low conductivity buffer designed to replace tris/glycine (Towbin) buffer in wet (tank) transfer applications. It allows protein transfer from gels to nitrocellulose or PVDF at relatively higher voltages.The speed of transfer of a protein from the gel to the membrane during a western blot is proportional to the electric field strength. It is directly proportional to the voltage applied and inversely proportional to the distance between the electrodes and is expressed in V/cm.  The amount of heat produced during the transfer is a function of the current flowing through the system. A lower conductivity buffer like ProtoBlot can be run at a higher voltage producing less heat because the current conducted will be lower. This allows for a shorter run time and more efficient transfer.Key notes:For use the 10X buffer is diluted to 1X and ethanol or methanol is added to 10%-20%.The pH of the buffer at a 1X dilution is 8.4–8.5 which is the ideal pH for maximum transfer of most proteins.The transfer can be done with or without an ice pack or cooling system.Optimum transfer times will vary and have to be determined empirically, but a suggested starting point is 300V for 20 minutes.