Golgicide A

ADP-ribosylation factor 1 (Arf1) plays an important role in regulating secretory traffic and membrane transport within the Golgi. Arf1 is activated by guanine nucleotide exchange factors (ArfGEFs). Golgicide A is a potent, specific and reversible inhibitor of the cis-Golgi ArfGEF GBF1. Inhibition of GBF1 function results in rapid dissociation of COPI vesicle coat from Golgi membranes and subsequent disassembly of the Golgi and trans-Golgi network (TGN). Golgicide A inhibits the effect of shiga toxin on protein synthesis with an IC₅₀ of 3.3 μM in Vero cells.