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TMB (dihydrochloride x.hydrate)

TMB (dihydrochloride x.hydrate) is the dihydrochloride x hydrate form of TMB. TMB (dihydrochloride x.hydrate) is a non-toxic, non-mutagenic, colorless dye that is also used as a chromogenic agent in enzyme detection systems based on horseradish peroxidase (HRP). TMB (dihydrochloride x.hydrate) can function as a photoacoustic probe for reactive oxygen and nitrogen species (ROS/RNS), reacting with hypochlorite, hydrogen peroxide, singlet oxygen, and nitrogen dioxide to produce blue oxidation products. TMB (dihydrochloride x.hydrate) can detect the singlet oxygen produced by the outer membrane of Escherichia coli ATCC 25922[1][2].

Product Specifications

CAS Number

207738-08-7

Product Name Alternative

Sure Blue TMB (dihydrochloride x.hydrate)

UNSPSC

12352200

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye; Reactive Oxygen Species (ROS)

Type

Biochemical Assay Reagents

Related Pathways

Immunology/Inflammation; Metabolic Enzyme/Protease; NF-κB; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/tmb-dihydrochloride-x-hydrate.html

Purity

98.52

Solubility

DMSO : 10 mg/mL (ultrasonic)

Smiles

NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C.[x].Cl.Cl.O

Molecular Formula

C16H22Cl2N2.xH2O

Molecular Weight

331.29

Precautions

H302, H315, H319, H335

References & Citations

[1]Bresolí-Obach R, et al. Tetramethylbenzidine: An Acoustogenic Photoacoustic Probe for Reactive Oxygen Species Detection. Sensors (Basel) . 2020 Oct 21;20 (20) :5952. |[2]Frey A, et al. A stable and highly sensitive 3,3',5,5'-tetramethylbenzidine-based substrate reagent for enzyme-linked immunosorbent assays. J Immunol Methods. 2000 Jan 13;233 (1-2) :47-56.

Shipping Conditions

Room Temperature

Storage Conditions

Store at room temperature, keep dry and cool

Scientific Category

Biochemical Assay Reagents

Clinical Information

No Development Reported

Available Sizes

Frequently Asked Questions

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