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Acridine Orange base

Acridine Orange base is a cell-permeable fluorescent dye that stains organisms (bacteria, parasites, viruses, etc.) bright orange and, when used under appropriate conditions (pH=3.5, Ex=460 nm), distinguishes human cells in green for detection by fluorescence microscopy. Acridine Orange base fluoresces green when bound to dsDNA (Ex=488, Em=520-524) and red when bound to ssDNA (Ex=457, Em=630-644) or ssRNA (Ex=457, Em=630-644), also can be used in cell cycle assays[1][2][3].

Product Specifications

CAS Number

[494-38-2]

UNSPSC

12171500

Hazard Statement

H302

Target

Parasite

Type

Dye Reagents

Related Pathways

Anti-infection

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/acridine-orange-base.html

Purity

96.58

Solubility

1 M HCl : 200 mg/mL (ultrasonic) |DMSO : 35 mg/mL (ultrasonic) |H2O : 5.83 mg/mL (ultrasonic; warming; heat to 60°C)

Smiles

CN(C)C1=CC=C2C(N=C3C=C(N(C)C)C=CC3=C2)=C1

Molecular Formula

C17H19N3

Molecular Weight

265.36

Precautions

H302

References & Citations

[1]Darzynkiewicz Z, et al. Differential staining of DNA and RNA. Curr Protoc Cytom. 2004 Nov; Chapter 7:Unit 7.3.|[2]Mirrett S. Acridine orange stain. Infect Control. 1982 May-Jun;3 (3) :250-2. |[3]Yektaeian N, et al. Lipophilic tracer Dil and fluorescence labeling of acridine orange used for Leishmania major tracing in the fibroblast cells. Heliyon. 2019 Dec 18;5 (12) :e03073.

Shipping Conditions

Room Temperature

Storage Conditions

4°C (Powder, protect from light)

Scientific Category

Dye Reagents

Clinical Information

No Development Reported

Citation 01

Chemosphere. 2023 Apr:320:138071.|Research Square Print. December 12th, 2022.|ACS Appl Mater Interfaces. 2025 Feb 19;17 (7) :10446-10456.|Acta Pharm Sin B. 2021 Oct;11 (10) :3178-3192.|Adv Funct Mater. 2023 Apr 14.|Adv Sci (Weinh) . 2024 Aug 9:e2404693.|Anal Chem. 2025 Jul 15;97 (27) :14512-14520.|Biomaterials. 2025 May 17:323:123415.|Cell Death Dis. 2020 Jul 30;11 (7) :607.|Cell Death Dis. 2021 Jan 13;12 (1) :80.|Cell Mol Immunol. 2025 Mar;22 (3) :282-299.|Cell Prolif. 2022 Jan;55 (1) :e13172.|Cell Stress Chaperones. 2025 Sep 17:100112.|Clin Transl Med. 2023 Mar;13 (3) :e1229.|Ecotoxicol Environ Saf. 2025 Sep 3:303:119000.|Environ Toxicol. 2023 Sep;38 (9) :2100-2110.|Eur J Med Chem. 2024 Dec 5:279:116866.|Eur J Med Chem. 2024 May 17:273:116507|Free Radic Biol Med. 2023 Nov 1:208:402-417.|Int J Mol Med. 2023 May;51 (5) :43.|Int J Mol Sci. 2023 Sep 7;24 (18) :13812.|Invest Ophthalmol Vis Sci. 2025 Feb 3;66 (2) :5.|J Adv Res. 2025 Nov 7:S2090-1232 (25) 00877-X.|J Control Release. 2024 Dec:376:167-183.|J Dent Sci. 2025 Jul;20 (3) :1782-1791.|J Nutr Biochem. 2025 Aug 20:110082.|Microbiol Spectr. 2023 Jun 15;11 (3) :e0313822.|Nat Commun. 2023 Jun 30;14 (1) :3877.|Pharmaceuticals (Basel) . 2025 Nov 11;18 (11) :1714.|Signal Transduct Target Ther. 2025 Mar 19;10 (1) :94.|SSRN. 2023 Mar 9.|SSRN. 2023 Nov 15.|STAR Protoc. 2024 Sep 12;5 (3) :103309.

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