Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

Recombinant Human APE

Product Specifications

Background

Apurinic-Apyrimidinic Endonuclease 1 (APE1) is required for efficient DNA base excision repair. When the DNA glycosylase remove the damaged bases, APE1 cleaves the AP site to allow resynthesis and ligation to complete repair. APE1 stimulates the DNA binding activity of many transcription factors, which participate in cancer promotion and progression. APE1 regulates the redox state of multiple transcription factors, such as c-Jun, c-Fos, NF-kB, p53. APEN is also involved in calcium-dependent down-regulation of PTH expression.

Product Name Alternative

DNA- (Apurinic or Apyrimidinic Site) Lyase; APEX Nuclease; APEN; Apurinic-Apyrimidinic Endonuclease 1; AP Endonuclease 1; APE-1REF-1; Redox Factor-1; APEX1; APE; APE1; APEX; APX; HAP1; REF1

Accession Number

AAH02338.1

Tag

Tag free

Source

E.coli

Endotoxin

<1 EU/μg as determined by LAL test.

Purity

>95% as determined by reducing SDS-PAGE.

Form

Liquid

Molecular Weight

35.62 KDa

Shipping Conditions

The product is shipped on dry ice/polar packs. Upon receipt, store it immediately at the temperature listed below.

Storage Conditions

Store at ≤-70°C, stable for 6 months after receipt. Store at ≤-70°C, stable for 3 months under sterile conditions after opening. Please minimize freeze-thaw cycles and use a manual defrost freezer.

Protein Length

Pro2-Leu318

Formulation

Supplied as a 0.2 μm filtered solution of 10mM HEPES, 100mM KCl, 50% Glycerol, pH 7.4.

Species

Human

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