Secondary Antibody Dilution Buffer

<strong>Secondary Antibody Dilution Buffer</strong>_x000D_ <table width="1028">_x000D_ <tbody>_x000D_ <tr>_x000D_ <td width="285">Catalog number:</td>_x000D_ <td width="743">B2010081</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Lot number:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Expiration date:</td>_x000D_ <td>Batch Dependent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Volume/Weight</td>_x000D_ <td>50 mL</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>pH</td>_x000D_ <td>7.4</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Supplied as</td>_x000D_ <td>Ready-to-use solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Appearance</td>_x000D_ <td>clear solution</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Applications</td>_x000D_ <td>General dilution buffer for secondary antibodies used in ELISA and similar immunoapplications. Buffer is compatible with HRP conjugates.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Shelf-Life</td>_x000D_ <td>1 year from date of manufacture</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Storage:</td>_x000D_ <td>Keep at 2-8°C</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Keywords:</td>_x000D_ <td>Secondary antibody diluent, antibody conjugate diluent, HRP diluent</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Grade</td>_x000D_ <td>Biotechnology grade. All components are highly pure (minimum 99%). All solutions are made with Type I ultrapure water (resistivity &gt;18 MΩ-cm) and are filtered on a 0.22 um.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>References</td>_x000D_ <td width="743">1: Ge M, Li RC, Qu T, Gong W, Yu XL, Tu C. Construction of an HRP-streptavidin_x000D_ bound antigen and its application in an ELISA for porcine circovirus 2_x000D_ antibodies. AMB Express. 2017 Sep 18;7(1):177. doi: 10.1186/s13568-017-0473-3._x000D_ PMID: 28921455; PMCID: PMC5603472.2: Poolperm P, Varinrak T, Kataoka Y, Tragoolpua K, Sawada T, Sthitmatee N._x000D_ Development and standardization of an in-house indirect ELISA for detection of_x000D_ duck antibody to fowl cholera. J Microbiol Methods. 2017 Nov;142:10-14.3: Panda R, Boyer M, Garber EAE. A multiplex competitive ELISA for the detection_x000D_ and characterization of gluten in fermented-hydrolyzed foods. Anal Bioanal Chem._x000D_ 2017 Dec;409(30):6959-6973._x000D_ 4: Jadhav PD, Shim YY, Reaney MJ. Synthesis and Characterization of Site-_x000D_ Selective Orbitide-BSA Conjugate to Produce Antibodies. Bioconjug Chem. 2016 Oct_x000D_ 19;27(10):2346-2358._x000D_ _x000D_ 5: Kim WJ, Cho HY, Jeong B, Byun S, Huh J, Kim YJ. Synergistic Use of Gold_x000D_ Nanoparticles (AuNPs) and "Capillary Enzyme-Linked Immunosorbent Assay (ELISA)"_x000D_ for High Sensitivity and Fast Assays. Sensors (Basel). 2017 Dec 26;18(1):55._x000D_ _x000D_ 6: Mitsui Y. Development of a simple and specific direct competitive ELISA for_x000D_ the determination of artesunate using an anti-artesunate polyclonal antiserum._x000D_ Trop Med Health. 2016 Nov 21;44:37._x000D_ _x000D_ 7: Al-Dujaili EA, Baghdadi HH, Howie F, Mason JI. Validation and application of_x000D_ a highly specific and sensitive ELISA for the estimation of cortisone in saliva,_x000D_ urine and in vitro cell-culture media by using a novel antibody. Steroids. 2012_x000D_ May;77(6):703-9._x000D_ _x000D_ 8: Al-Dujaili EA. Development and validation of a simple and direct ELISA method_x000D_ for the determination of conjugated (glucuronide) and non-conjugated_x000D_ testosterone excretion in urine. <a href="https://pubmed.ncbi.nlm.nih.gov/16098502/">Clin Chim Acta</a>. 2006 Feb;364(1-2):172-9.</td>_x000D_ </tr>_x000D_ <tr>_x000D_ <td>Products Related to Secondary Antibody Dilution Buffer</td>_x000D_ <td><a href="https://moleculardepot.com/product-category/buffers/">Biochemical Buffers and Solutions</a></td>_x000D_ </tr>_x000D_ </tbody>_x000D_ </table>