Anti-CA-125/MUC16 Antibody

Mouse Monoclonal Antibody specific to CA-125

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

MUC-16, Ovarian cancer-related tumor marker CA125, CA-125, Ovarian carcinoma antigen CA125

Gene Name

MUC16

Gene ID

94025

NCBI Gene ID

UniProt

Q8WXI7

Accession Number

NP_078966

Cellular Locus

Cell membrane; Single-pass type I membrane protein. Secreted, extracellular space. Note=May be liberated into the extracellular space following the phosphorylation of the intracellular C-terminus which induces the proteolytic cleavage and liberation of the extracellular domain.

Host

Mouse

Reactivity

Human

Immunogen

CA-125 purified from ovarian carcinoma cells.

Target Antigen

Mucin-16

Target

CA-125/MUC16

Clonality

Monoclonal

Isotype

IgG1

Type

Antibody

Applications

WB, ELISA, IHC

Field of Research

Cancer Research

Purification Method

Purified by ion exchange chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium that is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Tris Buffer

Function

Thought to provide a protective, lubricating barrier against particles and infectious agents at mucosal surfaces. {ECO:0000250}.

Additionnal Information

These antibodies may be used in ELISA and Western blotting to detect CA-125. <br><br>Positive control: OVCAR3 ovarian adenocarcinoma cells.Suggested pairs for sandwich ELISA:

Storage Conditions

These antibodies are stable for at least one (1) year at -20°C. Avoid multiple freeze-thaw cycles.

Specificity

#3330 and #3345 epitope group B (ISOBM* classification). #3346 epitope group A (ISOBM* classification)*International Society of Oncology and Biomarkers

Formulation

10mM Tris buffer, pH 7.5, 0.15M NaCL, 0.05% sodium azide

Buffer pH

pH 7.5

Target Background

CA-125 (cancer antigen 125, carcinoma antigen 125, or carbohydrate antigen 125) also known as mucin 16 or MUC16 is encoded by the MUC16 gene in humans. MUC16 is a member of the mucin family of glycoproteins. CA-125 is most consistently elevated in epithelial ovarian cancer, but can be expressed in a number of gynecologic (endometrial, fallopian tube) and non- gynecologic (pancreatic, breast, colon and lung) cancers. It may also be elevated in cases of benign abnormalities such as endometriosis.