Recombinant Eucheuma serra Lectin ESA-2

GRYTVQNQWGGSSAPWNDAGLWILGSRGNQNVMAVDVNSSDGGANLNGTMTYSGEGPIGFKGARRGESNVYDVENQWGGSSAPWHAGGQFVIGSRSGQGVLAVNITSSDGGKTLTGTMTYEREGPIGFKGTQSGGDTYNVENQWGGSSAPWNKAGIWALGDRSGQAMIAMDVSSSDGGKTLEGTMQYKGEGPIGFRGKLSGANNYSVENQWGGSSAPWNAAGDWLIGDRHNQNITAVKVSSDNDGKNLDGTCTYEREGPIGFKGVATS

Lectin specific for high mannose N-glycans, recognizes the branched moiety of these glycans. Does not recognize other types of N-glycans or monosaccharides. Agglutinates trypsin-treated sheep and rabbit erythrocytes and untreated sheep erythrocytes. Has mitogenic activity on mouse lymphocytes. Does not require metal ions for activity.

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

"Strict specificity for high-mannose type N-glycans and primary structure of a red alga Eucheuma serra lectin." Hori K., Sato Y., Ito K., Fujiwara Y., Iwamoto Y., Makino H., Kawakubo A. Glycobiology 17:479-491 (2007)

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.