Mouse anti Human CD56, conjugated with PerCP
Selected Variant
0565
Size: 100 Tests
Product Specifications
Background
CD56, M.W. 150,000, is expressed on approximately 10-25% of Human peripheral blood lymphocytes. CD56 (NKH-1) is expressed on Human peripheral blood natural killer cells, representing a pan NK-cell antigen. Expressed on non-MHC-restricted cytotoxic T cells. Exalpha's CD56, clone C5.9 has been recognized as the superior CD56 antibody for flow cytometry applications because of its specific and intense staining.
CAS Number
9007-83-4
Synonyms
CD56, NCAM1, Neural Cell Adhesion Molecule
UniProt
P13591
Host
Mouse
Species Reactivity
Human
Isotype
IgG2b
Clone
C5.9
Type
Single-Color Reagent
Source
Derived from the hybridization of mouse Sp2/0 myeloma cells with spleen cells from BALB/c F1 mice immunized with KG1a cell line.
Applications
Flow Cytometry, Direct IF
Field of Research
Flow Cytometry
Purification Method
Protein A/G Chromatography
Assay Principle
PBMC: Add10 µL of MAB/10^6 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2º to 8º C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature 20ºC. Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturer’s instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope.
Stability
See expiration date on vial
Concentration
Product provided titered for use in flow cytometry when used as indicated
Form
PerCp conjµgated monoclonal antibody C5.9, provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein
Precautions
This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.
Shipping Conditions
Room Temperature
Storage Conditions
Product should be stored at 4-8ºC; DO NOT FREEZE
Functional Analysis
Flow Cytometry Staining
Price
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