DR3:TL1A Inhibitor Screening Assay Kit

DR3 (death receptor 3), also known as tumor necrosis factor receptor superfamily member 25 or TNFRSF25, is a membrane receptor of the tumor necrosis factor receptor superfamily of proteins (TNFRSF), which associates with TL1A (TNF-like protein 1A) in T and NK cells. DR3 has been recognized as a significant anti-apoptotic and differentiation factor and it is a co-stimulatory receptor. TL1A, also called TNFSF15, is a member of the tumor necrosis factor family. It is expressed in different immune cells, such as monocyte, macrophage, dendritic cell, T cell and non-immune cells. TL1A competitively binds to DR3, having a higher affinity for DcR3 (decoy receptor 3), providing stimulatory signal for downstream signaling pathways. It then regulates proliferation, activation, apoptosis, and chemokine production in effector cells. The role of DR3 in T cell activation, and consequently in cytokine secretion and cell proliferation, makes it an attractive target in cancer therapy. Inhibition of DR3-TL1A interaction has substantial therapeutic potential in the treatment of solid tumors.

Screen or titrate small molecule inhibitors or biologics for drug discovery and high-throughput screening (HTS) applications of the TL1A binding to DR3.

96 ReactionsCatalog #NameAmountStorage101882TL1A, His-Tag, Avi-Tag, Biotin-Labeled*5 µg-80 °C102303DR3, His-Tag*25 µg-80 °C826205x PP-02 Buffer4 ml-20 °C82719Blocking Buffer 740 ml+4 °C79742Streptavidin-HRP10 µl+4 °C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7969996-well white microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.384 ReactionsCatalog #NameAmountStorage101882TL1A, His-Tag, Avi-Tag, Biotin-Labeled*2 x 5 µg-80 °C102303DR3, His-Tag*2 x 25 µg-80 °C826205x PP-02 Buffer4 ml-20 °C82719Blocking Buffer 72 x 40 ml+4 °C79742Streptavidin-HRP2 x 10 µl+4 °C79670ELISA ECL Substrate A (translucent bottle) 2 x 6 mlRoom TempELISA ECL Substrate B (brown bottle) 2 x 6 mlRoom Temp78188384-well white microplate1Room TempPlate sealing film1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

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