CDK9/CyclinT Kinase Assay Kit

Cyclin-dependent kinase 9 (CDK9) is the catalytic subunit of the positive transcription elongation factor b (P-TEFb) complex, which phosphorylates the C-terminal domain of RNA polymerase II, a key player in the production of mature RNA. P-TEFb also includes cyclinT. CDK9 translocates to the nucleus once it gets auto phosphorylated, where it can associate with cyclinT. Hyperactivation of cyclins is known to result in cancer and the development of resistance to therapy, and CDK9-cyclinT is involved in the transcription of oncogenes. The inhibition or degradation of specific CDK-cyclin complexes could prove beneficial in cancer treatment, but the development of specific inhibitors has been difficult. Recently a small molecule degrader specific for CDK9-cyclinT was identified, LL-K9-3, which resulted in lower levels of androgen receptor (AR) and cMyc in 22RV1 cells. LL-K9-3 thus seemed more effective than SNS032, its parental CDK9 inhibitor, and the CDK9 PROTAC Thal-SNS032. Further research into new and more specific degrader molecules will prove beneficial for cancer therapy.

96 reactionsCatalog #ComponentAmountStorage40307CDK9/CyclinT, GST-Tag*10 µg-80°C793345x Kinase Buffer 11.5 ml-20°C79686500 µM ATP100 µl-20°C796045x CDK substrate peptide 21 ml-20°C7969696-well plate, white1Room temp*The concentration of the protein is lot-specific and will be indicated on the tube.384 reactionsCatalog #ComponentAmountStorage40307CDK9/CyclinT, GST-Tag*2 x 10 µg-80°C793345x Kinase Buffer 12 x 1.5 ml-20°C79686500 µM ATP200 µl-20°C796045x CDK substrate peptide 22 x 1 ml-20°C79969White, 384-well plate1Room temp*The concentration of the protein is lot-specific and will be indicated on the tube.

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