Cathepsin L Inhibitor Screening Assay Kit

Cathepsin L is a lysosomal cysteine protease that belongs to the papain-like superfamily. It is involved in many essential physiological processes, including degradation and renewal of intracellular proteins, activation of prohormones, presentation of antigens, as well as organ development. One of its main functions is in the degradation of antigens, and it has been shown to participate in SARS-CoV-1 and SARS-CoV-2 infection, as infection requires viral membrane fusion and that seems to require proteolysis of the Spike protein by Cathepsin L. The use of amantadine inhibited Cathepsin L and prevented SARS-CoV-2 infection. Cathepsin L plays important roles in tumor metastasis and chemotherapy resistance, and upregulation of this enzyme has been reported in a wide range of human cancers including ovarian, renal, and breast carcinoma. The development of inhibitors targeting this protein may prove beneficial for both COVID-19 and cancer therapy.

96 reactionsCatalog #NameAmountStorage80005Cathepsin L, His-Tag*˃1 µg-80°C80349Fluorogenic Cathepsin Substrate 1 (5 mM) 10 µl-20°C4x Cathepsin Buffer2 ml-20°C0.5 M DTT200 µl-20°C1 mM E-6410 µl-20°C7968596-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.384 reactionsCatalog #NameAmountStorage80005Cathepsin L, His-Tag*1 µg-80°C80349Fluorogenic Cathepsin Substrate 1 (5 mM) 2 x 10 µl-20°C4x Cathepsin Buffer2 x 2 ml-20°C0.5 M DTT2 x 200 µl-20°C1 mM E-6410 µl-20°C79961384-well black microplate1Room Temp* The concentration of protein is lot-specific and will be indicated on the tube containing the protein.

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]