WRN Helicase Activity Assay Kit

The WRN Helicase Activity Assay is a fluorogenic assay designed for screening and profiling of WRN (Werner Syndrome ATP-dependent Helicase) antagonists/inhibitors by monitoring their effect on WRN-catalyzed DNA unwinding. WRN Helicase Activity Assay Kit comes in a convenient 96-well or 384-well format, which contains enough purified recombinant WRN, ATP, DNA substrate, assay buffer and additives for 100 or 400 reactions.Figure 1: Illustration of the assay principle.The DNA probe is conjugated on one strand with the TAMRA (tetramethylrhodamine) fluorophore, and on the other strand with BHQ (Black Hole Quencher) which effectively quenches TAMRA fluorescence due to their proximity within the DNA double strand. WRN unwinding of the DNA probe separates the two strands, releasing TAMRA fluorescence. WRN activity, therefore, results in a proportional increase in fluorescence.Need us to run inhibitor screens or profile your compounds against WRN? Check out ourDNA Replication & Repair Screening Services.

Product Specifications

Background

The WRN gene was first discovered as the gene mutated in Werner syndrome, a recessive genetic disorder characterized by segmental progeria and elevated cancer predisposition. WRN is a multifunctional enzyme with helicase and exonuclease activities and plays roles in various cellular processes crucial for the maintenance of genome stability, including DNA replication, transcription, DNA repair, and telomere maintenance. WRN depletion causes cell cycle arrest, DNA damage, mitotic defects, chromosome shattering, and apoptosis. Loss of heterozygosity involving theWRNloci at chromosome 8p11.2-p12 occurs frequently in many different cancers, pointing to its role as a tumor suppressor gene. Small molecule inhibitors of WRN can be used to induce synthetic lethality and offer a new therapeutical approach for cancer treatment.

UniProt

Q14191

Applications

Screen small molecule inhibitors or antagonists that affect helicase activity of WRN in high throughput screening (HTS) applications.

Format

96 reactionsCatalog #NameAmountStorage101264WRN (517-1093), GST-Tag*2 x 10 µg-80°C825474x WRN Buffer4 ml-20°C82645WRN Substrate20 µl-80°CATP (200 mM) 50 µl-20°C0.5 M DTT200 µl-20°C79685Black, low binding plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.384 reactionsCatalog #NameAmountStorage101264WRN (517-1093), GST-Tag*4 x 10 µg-80°C825474x WRN Buffer2 x 4 ml-20°C82645WRN Substrate2 x 20 µl-80°CATP (200 mM) 2 x 50 µl-20°C0.5 M DTT2 x 200 µl-20°C79961Low binding, black 384-well plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For further questions, please email[email protected]

Contraindications

The final concentration of DMSO in the reaction should not exceed 1%.Compounds that are fluorescent may interfere with the results, depending on their spectral excitation and emission properties.It is recommended that the compound alone is tested to determine any potential interference of the compound with the assay results.