Chemi-Verse™ PARP7 Assay Kit

The Chemi-Verse™ PARP7 Assay Kit is designed to measure the activity of PARP7 (poly- (ADP-ribose) polymerase 7) for screening and profiling applications. The PARP7 assay kit comes in a convenient 96-well or 384-well format, with enough recombinant purified PARP7 enzyme (amino acids 400-657), histone mixture, NAD+, a ribose-binding agent and PARP assay buffer for 100 or 400 enzyme reactions.Figure 1. Chemi-Verse™ PARP7 Assay Kit schematic.Histone proteins are coated on a 96-well or 384-well plate. Next, a biotinylated NAD+substrate is incubated with the PARP7 enzyme in an optimized assay buffer. The plate is then treated with an ADP-ribose specific binding agent and a secondary HRP-conjugated antibody. ELISA ECL substrate is added to produce chemiluminescence that can be measured using a chemiluminescence reader. The chemiluminescence signal is proportional to PARP7 activity.Need us to run inhibitor screens or profile your compounds against PARP7? Check out ourPARP/PARPTrap™ Screening ServicesorDNA Replication and Repair Screening Services.

Product Specifications

Background

PARP7, also known as poly- (ADP-ribose) polymerase 7 or NAD+ADP-ribosyltransferase 7, is part of the PARP family. ADP ribosylation, which is the addition of an ADP-ribose to a protein, is a reversible post-translational modification of proteins mostly involved in the DNA Damage Response (DDR) pathway. Mono-ADP-ribosylation (termed MARylation) is the addition of a unit of ADP-ribose. PARP7 is a negative regulator of nucleic acid sensing in cancer cells. Tumor cells contain cytosolic DNA as a reflection of defects in DNA repair and must repress responses coming from the adaptative immune system. PARP7 is an AHR (aryl hydrocarbon receptor) regulated gene that inhibits STING (stimulator of interferon genes) and IFN-I (interferon type I) . RBN-2397 is a pyridazinone-based PARP7 inhibitor that results in STING-dependent and IFN-I activation and leads to cancer cell deathin vitroandit is currently under clinical trial. The development of PARP7 inhibitors is thus a promising area in cancer research.

UniProt

Q7Z3E1

Applications

Study enzyme kinetics and screen small molecule inhibitors for drug discovery and high throughput screening (HTS) applications.

Format

96 reactionsCatalog #NameAmountStorage80527PARP7, FLAG-Tag*2 µg-80°C520295x Histone Mixture1 ml-80°C50 µM NAD+250 µl-80°CPP-16-2 Assay Buffer1 ml-20°C79743Blocking Buffer 32 x 25 ml+4°C0.5 M DTT200 µl-20°CRibose-Binding Reagent 2-D10 µl-80°C52131HSecondary HRP-Antibody 210 µl-80°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7983796-well module plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube384 reactionsCatalog #NameAmountStorage80527PARP7, FLAG-Tag*2 x 2 µg-80°C520295x Histone Mixture2 x 1 ml-80°C50 µM NAD+2 x 250 µl-80°CPP-16-2 Assay Buffer2 x 1 ml-20°C79743Blocking Buffer 32 x 25 ml+4°C0.5 M DTT2 x 200 µl-20°CRibose-Binding Reagent 2-D2 x 10 µl-80°C52131HSecondary HRP-Antibody 22 x 10 µl-80°C79670ELISA ECL Substrate A (translucent bottle) 2 x 6 mlRoom TempELISA ECL Substrate B (brown bottle) 2 x 6 mlRoom Temp78188384-well white plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube

Shipping Conditions

-80°C

Storage Conditions

This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

Notes

Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]