MAT2A Inhibitor Screening Assay Kit

MAT2A (methionine adenosyltransferase 2A), also known as AdoMet Synthase 2, is the rate limiting enzyme in the methionine cycle, where it catalyzes the formation of S-adenosylmethionine (SAM) from methionine and ATP. It is the catalytic subunit of the S-adenosylmethionine synthetase complex. The levels of SAM are crucial for many cellular processes, including the modification of DNA, RNA and histones.  In liver, MAT2a expression is associated with growth, dedifferentiation, and cancer. In CRPC (castration-resistant prostate cancer) it plays a role in maintaining the cancer stem cell stemness and tumorigenesis, potentially by promoting H3K4me2 of pro-tumorigenic non-canonical AR (androgen receptor) targets. The use of MAT2A inhibitors showed promise in CRPC models, when combined with AR and EZH2 (enhancer of zeste homolog 2) inhibitors. It is a synthetic lethal target in MTAP (methylthioadenosine phosphorylate) -deficient cancers, making it a promising oncotherapy target.

96-WellCatalog #NameAmountStorage71401MAT2A, His-Tag*125 µg-80°C82188ATP (750 µM) 0.5 ml-20°C82189L-Methionine (750 µM) 0.5 ml-20°C788695x MAT2A Assay Buffer3 ml-20°C74001Colorimetric Detection Reagent**10 ml+4°C82770Transparent, half-volume 96-well plate1Room Temp.384-WellCatalog #NameAmountStorage71401MAT2A, His-Tag*250 µg-80°C82188ATP (750 µM) 1 ml-20°C82189L-Methionine (750 µM) 1 ml-20°C788695x MAT2A Assay Buffer2 x 3 ml-20°C74001Colorimetric Detection Reagent**2 x 10 ml+4°C79962Transparent, 384-well plate1Room Temp.*The concentration of the protein is lot-specific and will be indicated on the tube.**Colorimetric Detection Reagent is used to measure the free phosphate from the MAT2A reaction. Any source of inorganic phosphate can interfere with the assay.

The final concentration of DMSO in the assay should not exceed 1%.Some compounds may interfere with the results, depending on their spectral excitation and emission properties.It is recommended that the compound alone is tested to determine any potential interference of the compound with the assay results.Colorimetric Detection Reagent is used to measure the free phosphate from the MAT2A reaction. Any source of inorganic phosphate can interfere with the assay.