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FluoSite™ Anti-PD-1 Neutralizing Antibody, PE-labeled

R-Phycoerythrin-labeled anti-PD-1 neutralizing recombinant murine/human chimeric antibody recognizing the PD-L1/PD-L2 binding region of human PD-1.FluoSite™is a tag-based method for site-specific conjugation of antibodies with fluorophores, ensuring the labeling site is far from the antigen-binding domain to preserve antibody functionality. This precise, consistent labeling ensures the homogeneity of the labeled antibody preparation, making it ideal for flow cytometry and other high-sensitivity applications.

Product Specifications

UniProt

Q15116

Cross Reactivity

This antibody does not cross-react with mouse PD-1, but does cross-react with monkey (M. fascicularis) PD-1. It has not been tested with other species.

Label

R-Phycoerythrin (PE) is an oligomeric protein complex (270 kDa) from red algae that exhibits intensely bright red-orange fluorescence with high quantum yields. The complex consists of six heterodimers, α subunit (18 kDa) and β-subunit (20 kDa), and an additional γ-subunit (34 kDa) . PE is covalently attached randomly through lysines on the target protein.

Purification

Protein A affinity chromatography from HEK293 supernatants.

Purity

≥ 90%

Shipping Conditions

-80°C

Storage Conditions

At least 12 months at -80°C.

Calculated Molecular Weight

Heavy Chain: 49 kDa + glycans + PE, Light Chain: 23 kDa + PE

Formulation

8 mM phosphate pH 7.4,110 mM NaCl, 2.2 mM KCl, 20% glycerol

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